Shimadzu

 expands the product line and improves performance and user-friendliness. With i-Series Plus, pretreatment operations have been automated, such as the processes to dilute samples and add reagents. This results in better reproducibility and measurement accuracy by minimizing human error, to ensure reliable data in the analysis of pharmaceuticals and foods. 

 also offers new features that satisfy data integrity requirements of the pharmaceutical industry. The 

 consists of the compact HPLC Prominence-i and UHPLC system Nexera-i.

Articles

The i-Series Plus, designed for covering conventional to ultra-fast LC applications, consists of the compact HPLC Prominence-i and UHPLC system Nexera-i. High speed analysis, simplified method transfer, minimized environmental impact and easy maintenance are just a few of the many new features of this product range

i-Series Plus integrated HPLCSignificantly improved analytical productivity with automated pretreatment operations

 is the latest in a long line of MALDI-TOF products from Shimadzu. This benchtop, linear MALDI-TOF mass spectrometer delivers outstanding performance in a compact footprint, making it an ideal choice for today’s increasingly demanding laboratories.


	   •    Linear mode (positive ion) MALDI-TOF
	   •    200 Hz solidâstate laser, 355 nm
	   •    Load-lock chamber for fast sample introduction
	   •    UV laserâbased source cleaning (patented)
	   •    Small size due to benchtop design with a compact footprint
	   •    Quiet operation (<55 dB)

	Linear mode instruments have traditionally been the instruments of choice in MALDI-TOF-based quality control (QC) and profiling workflows, and the 

 is no exception. Peptides, proteins, polymers or oligonucleotides â the instrument is capable of delivering the performance required for QC applications. Research laboratories will also benefit from the MS capabilities of the instrument through the rapid mass-measurement of samples.

	Albert-Hahn-Str. 6-10, 47269 Duisburg, Germany
	Phone: +49-203-76 87 0
	Fax: +49-203-76 66 25
	

MALDI-8020

	Celebrating this year the 50th anniversary of its presence in Europe, Shimadzu emphasizes its Excellence in Science approach through the release of the new LCMS-9030 quadrupole time-of-flight liquid chromatograph mass spectrometer. The LCMS-9030 Q-TOF system was launched in Europe at the IMSC in Florence, Italy. It highlights the company’s position of a world leader in analytical instrumentation and a fastest growing mass spectrometry company.

 is a research grade mass spectrometer designed to deliver high-resolution, accurate mass detection with incredibly fast data acquisition rates, allowing scientists to identify and quantify more compounds with greater confidence. It utilizes the same engineering DNA as Shimadzu’s proven, rugged, high-performance triple quadrupole (LC-MS/MS) platform and integrates this with powerful TOF architecture to transform high mass accuracy workflows by achieving high-sensitivity, high-speed and high-resolution detection.

 provides effortless performance through less need for calibration and easy switching between ionization units. It has been unveiled at the 66th ASMS Conference on Mass Spectrometry and Allied Topics from June 3-7 in San Diego, USA.


	Ultra-fast (UF) acquisition rates and core ion beam technologies developed for the triple quadrupole platform have created new possibilities in quantitative mass spectrometry by delivering exceptional sensitivity, specific quantitation and enhanced target compound verification. The new 

 Q-TOF builds on this proven platform by rethinking time-of-flight detection.

	In the LCMS-9030, core ion beam technologies transition towards a unique approach in ion gating using UFaccumulation

 to create a precise pulse of ions in the flight tube optimized for high sensitivity and high resolution using iRefTOF

 generates an ideal reflectron field, delivering the highest resolution for the flight path with highly stable mass accuracy.

New solution for analyzing even the most complex samples


	By bringing together UF technologies and innovative TOF thinking, the LCMS-9030 represents a step-change in Q-TOF performance and capability by unlocking a new solution for analyzing even the most complex samples. It integrates the world’s fastest and most sensitive quadrupole technology with TOF architecture.

 will push the boundaries further for high mass accuracy and high mass resolution detection, and will make an impact across all applications, from small molecule quantitation to complex intact protein analysis. This technology highlights Shimadzu’s passion for making something new and innovative as well as our commitment to making analysis better and promoting better science,” said Shuzo Maruyama, General Manager, Analytical & Measuring Instruments Division, Shimadzu Corporation.


	UFaccumulation and iRefTOF are registered trademarks of Shimadzu Corporation

LCMS-9030 Q-TOF: Effortless performance

	Shimadzu, a worldwide leading manufacturer of analytical instrumentation, provides perfect fit solutions in hardware and software components. For amino acid sequences, the 

 create user benefits leading to greater simplicity and reliability combined with FDA compliance.

 separate PTH-amino acids isocratically. This improves baseline stability and allows high-sensitivity analysis of PTH-amino acids. Isocratic sequence analysis provides more stable retention times, so peaks detected in previous cycles can be cancelled using subtraction chromatogram processing, making it easier to identify sequences.

 provides compliance with FDA 21 CFR Part 11 guidelines and enables compliance with the security, user management and audit trail requirements specified by FDA 21 CFR Part 11. Specialized protein sequencer software makes it simple to perform reprocessing of chromatograms, overlay of multiple chromatograms and automatic estimation of amino acid sequences, which are required for sequence analysis.

	Shimadzu Europa GmbH
	Albert-Hahn-Strasse 6-10
	47269 Duisburg, Germany
	phone: +49 (0) 203-7687-0
	

Greater simplicity and reliability in the determination of amino acid sequences

	Celebrating this year the 50th anniversary of its presence in Europe, Shimadzu emphasizes its Excellence in Science approach through the release of the new 

-2000 automated pretreatment module for cell culture media analysis. C2MAP-2000 highlights the company’ s position of a world leader in analytical instrumentation. This product is for pretreating of culture media samples by adding reagents such as a deproteinizing agent or internal standard substance, filtering of precipitated proteins, and diluting of samples.

-2000 module with a Shimadzu ultra-fast liquid chromatograph mass spectrometer (LC-MS/MS). By automating all the process steps from pretreatment to measurement, the C2MAP system shortens the culture media analysis time for up to 95 components in culture supernatants to one sixth of the previous level. In addition, this system can be operated by less experienced operators.

	After simply loading culture supernatant samples, reagents and pairs of filter and recovery vials, the C2MAP-2000 module is able to execute all steps from pretreatment to LC-MS/MS measurement without any human intervention. This seamless execution not only makes it easy to process samples unattended overnight or on non-working days, but also enables samples to be controlled using the same ID for all process steps. This minimizes the risk of selecting the wrong sample while ensuring traceability. 

-TRENDS time-course viewer software loads the measurement data obtained and graphs temporal changes in each component concentration as trend graphs.

Growing importance of LC-MS/MS analysis to optimize cell culture conditions


	In recent years in antibody pharmaceutical fields, it has become increasingly important to record the consumption of components and accumulation of waste matter (secreted metabolites) in culture media, so that cell cultivation parameters can be optimized for manufacturing drug substances. In addition, in regenerative medicine fields that involve iPS, ES or other cells, there is a need for technology that can evaluate cell quality in real time without damaging the cells. There is also a need to reduce the replacement frequency of expensive culture media. Since monitoring the changes in culture supernatant components over time provides information that is useful in these fields, the role of 

/MS analysis has increased in the optimization of cell culture conditions.

	However, due to the lack of products available for seamlessly performing of all processes from sample preparation to measurement, there was a need for a system that could be used easily, even by less experienced operators. Shimadzu therefore developed the 

 automated pretreatment module and the C2MAP system for steps involved in determining the status of cultures and researching cultivation process steps at major pharmaceutical companies, biotech ventures, pharmaceutical CMOs (contract manufacturing organizations) and various research institutions. In the future, Shimadzu intends to offer solutions based on 

/MS measurement technology for controlling and optimizing of cell cultivation process steps.


	C2MAP and C2MAP system are registered trademarks or trademarks of Shimadzu Corporation.